Methods for Detection of Glycosylated Hemoglobin

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Glycosylated hemoglobin A2 components.

Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glycohemoglobins was obtained by showing that 14C-glucose bound to hemoglobin A2 mo...

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Glycosylated Hemoglobin in Perspective

With each new tool the insurance industry obtains to evaluate both states of health and states of disease, the temptation exists to use it in as many contexts as possible. The initial understanding in the mid-1960’s that diabetic individuals have different percentages of specific hemoglobins has led to the study and refinement of the hemoglobin Alc (HbAlc), which is being used increasingly by c...

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Improved colorimetric assay for glycosylated hemoglobin.

This colorimetric assay for glycosylated hemoglobin can be performed in 120 min with equipment available in most clinical laboratories. The glucose moiety of glycosylated hemoglobin is converted to 5-hydroxymethylfurfural by heating with oxalic acid for 60 min is an autoclave at 124 degrees C and 124 kPa (18 lb/in.2). The adduct formed by reacting 2-thiobarbituric acid with hydroxymethylfurfura...

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Predictive factors of glycosylated hemoglobin using additive regression model

Introduction: Diabetes is a chronic disease, non-epidemic disease that costs a lot of money in each year. One of the diagnostic criteria for diabetes is Glycosylated Hemoglobin (HBA1C), which in this study the effective factors on it examined by additive regression model. Materials and Methods: In this cross-sectional study, 130 patients with diabetes type-2 were selected based on simple random...

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ژورنال

عنوان ژورنال: Advances in Analytical Chemistry

سال: 2017

ISSN: 2163-1557,2163-1565

DOI: 10.12677/aac.2017.73022